Research Achievements

August 18, 2017  PRESS RELEASE

How Whip-like Cell Appendages Promote Bodily Fluid Flow

Researchers at Nagoya University have identified a molecule that enables cell appendages called cilia to beat in a coordinated way to drive the flow of fluid around the brain; this prevents the accumulation of this fluid, which otherwise leads to swelling of the head as found in the condition hydrocephalus.

press released on August 18, 2017



Caption:  On the lateral ventricular wall, well-oriented cilia exhibit a coordinated beating motion that drives directional CSF flow. Daple mutant mice lose the orientation of ciliary bases, leading to stagnant CSF flow and hydrocephalus. © Maki Takagishi


Nagoya, Japan - Some cells in the body contain long thin structures called cilia on their surface, which exhibit a whip-like motion that promotes the flow of fluid past the cell. Although these cilia are known to play vital roles in the body, much remains to be understood about their molecular components and the mechanisms by which they work. This is especially true for the cilia on cells that line the ventricles of the brain, which contain cerebrospinal fluid (CSF) that has various functions including cushioning the brain against potentially damaging impacts.


A team at Nagoya University has shed light on this issue by revealing that a molecule called Daple is essential for cilia to adopt an arrangement by which they can beat in one direction at the same time, thereby creating a flow of fluid past the cell exterior. This arrangement on cell surfaces all along the lining of ventricles in the brain ensures the correct flow of CSF, which in turn prevents its accumulation associated with brain swelling known as hydrocephalus.   


The team revealed the importance of Daple by creating mutant mice that did not express the Daple protein. By around 20 days after birth, these mice had enlarged heads, similar to that in human hydrocephalus cases. Further studies showed that this was due to the flow of CSF being disrupted.


"We cut out part of the wall of the brain's lateral ventricle and investigated whether fluorescent beads would be propelled along its surface in a particular direction," says Maki Takagishi. "For mice with normal Daple expression, there was consistent movement in one direction, but this was absent in the Daple-knockout mice."


The findings also showed that the lack of Daple stopped cilia all adopting the same orientation on the same side of cells. Without the cilia all beating in the same direction, there would be no directional flow of CSF, leading to its accumulation and subsequent swelling.


According to Masahide Takahashi, "Daple functions through a cytoplasmic structure called microtubules, which are protein filaments involved in various functions including maintaining the overall structure of cells. When Daple is absent, the microtubules are unable to accurately specify the arrangement of structures called basal bodies, from which the cilia develop."


The team's findings should lead to a deeper understanding of diseases caused by the dysfunction of cilia. These include not only hydrocephalus, but also asthma and even female infertility, given the structural and functional similarities of cilia in the trachea and oviduct.



The article "Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus" was published in Cell Reports at




Maki Takagishi1, Masato Sawada4, Shinya Ohata5,6, Naoya Asai 1,7, Atsushi Enomoto1, Kunihiko Takahashi2, Liang Weng1, Kaori Ushida1Hosne Ara1, Shigeyuki Matsui2, Kozo Kaibuchi3, Kazunobu Sawamoto4,8, and Masahide Takahashi 1,7

1 Department of Pathology, Graduate School of Medicine, Nagoya University
2 Department of Biostatistics, Graduate School of Medicine, Nagoya University

3 Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University
4 Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University
5 Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo
6 Faculty of Pharmacy, Musashino University
7 Division of Molecular Pathology, Center for Neurological Disease and Cancer, Graduate School of Medicine, Nagoya University
8 Division of Neural Development and Regeneration, National Institute for Physiological Sciences




Related Links:

More details, News release by Graduate School of Medicine, Nagoya University


Graduate School of Medicine, Nagoya University



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This work was supported by a Grant-in-Aid for Japan Society for the Promotion of Science Fellows (13J40134 and 17K15593) to M. Takagishi and a Grant-in-Aid for Scientific Research (S) (26221304) to M. Takahashi; these grants were commissioned by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan.




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