Research Achievements

September 14, 2016  PRESS RELEASE

Japanese Research Team Elucidates Structure of Bacterial Flagellar Motor Protein

Nagoya University-led Japanese researchers reveal the 3D structure of a bacterial propeller protein.

press released on September 7, 2016

 

Caption: The three-dimensional structure of a complex of MotA and the flagellar motor structure in a bacterial cell. Many motile bacteria have rotating fiber (flagellum) generating from a cell surface which functions like a screw and create a driving force to move or swim. At the proximal end of flagellum there is a rotary motor which is composed of a rotor and a stator and ions, Na+ or H+, flow into cells by way of the stator. The flows of ions are converted into a rotational force by the interaction between the stator and the rotor. The three-dimensional structure of the MotA complex has been determined from a large number of electron microscope images in this report. © Michio Homma

 

Nagoya, Japan - Many bacterial species use spiral propellers (flagella) attached to motors to move through a liquid environment. An interaction between the rotor and stator components of the motor generates the rotational force required for movement. The stator converts electrochemical energy into mechanical force after undergoing a structural change caused by a movement of charged particles (ions) through an internal channel. Previous studies investigated the stator and its interaction with the rotor by constructing mutant proteins and analyzing their functions. However, little was known about stator structure.

 

A team of Japanese researchers led by Homma's laboratory of Nagoya University have now purified the stator protein MotA from a bacterium found in hot springs (Aquifex aeolicus) and analyzed its three-dimensional structure using electron microscopy mainly in cooperation with Namba's laboratory of Osaka University. They found that it can form a structure of four MotA molecules (called a tetramer), which differs in shape from the previously predicted complex. The study was recently published in Scientific Reports.

 

The MotA protein spans the bacterial membrane, and has previously been shown to form a tetramer complex with another transmembrane protein, MotB, creating the stator. In this latest work, MotA was expressed and purified from A. aeolicus, and found to be structurally stable. Assessment of its interactive potential revealed it can form a tetramer even in the absence of MotB.

 

Electron microscopy showed that the elongated top part of the MotA complex matches the size of the lipid bilayer of the bacterium, suggesting that it represents the transmembrane component. "This region has a globular shape that corresponds to a MotA tetramer fitted inside an aggregate of detergent molecules which were used to purify the protein," first author Norihiro Takekawa says.

 

The lower part of the complex has two arch-like regions with spiky projections. "These match the cytoplasmic domain of the MotA protein," corresponding author Michio Homma says. "We predict that its structure will change with the movement of ions through the stator channel and in association with the stator-motor interaction." The shape of the complex differs from that reported for a related protein complex in another bacterium.

 

*****

 

The article, "The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium" was published in Scientific Reports at DOI:10.1038/srep31526

 

*****

 

Authors: Dr. Norihiro Takekawa, Mr. Mizuki Gohara, Designated Assistant Prof. Yasuhiro Onoue, Associate Prof. Seiji Kojima, and Prof. Michio Homma, Graduate School of Science, Nagoya University, and their international research group including Osaka University, Kyushu University and Nagahara Institute of BioScience and Technology.

 

*****

Related Links:

Homma's Lab., Laboratory of Supramolecular Biology, Group of Biomembrane Functions, Division of Biological Science, Graduate School of Science, Nagoya University

 

NU Research, "Escherichia coil Powered by a Hybrid Engine: A Genetic Modification Allows a Hybrid-fuel Bacterial Flagellar Motor to Adapt to Environmental Change" (Mar. 25, 2014)

 

Media Coverage:

 EurekAlert!

 

 AlphaGalileo

 

 ResearchSEA

 

 Science Newsline, Physics & Chemistry

 

 Science Daily

 

 Phys.org

 

 Health Medicine Network

 

 Scicasts

*****

 

Funding: This work was supported in part by Grants-in-Aid for scientific research from the Japan Society for the Promotion of Science (24117004 and 23247024 to M.H., 26840035 to N.Te., 26650021, 24117001, 24117004 to T.K., and 25000013 to K.N.), by Platform for Drug Discovery, Informatics, and Structural Life Science from Japan Agency for Medical Research and Development (to A.H. and T.S.), and from the Japan Society for the Promotion of Science (13J02161 to N.Ta.). N.Ta. was partly supported by the Integrative Graduate Education and Research Program in Green Natural Sciences of Nagoya University.

 

Archive List
2017
2016
2015
2014
2013